New Delhi metallo-β-lactamase-1 (NDM-1), a carbapenemase represents a significant global public health threat and theorised to have originated from Acinetobacter species through the mobilisation of a Tn125. This study investigated the genetic mechanisms of blaNDM spread from Acinetobacter species to the Enterobacteriaceae through the complete sequencing of plasmids carrying blaNDM from Australian clinical Enterobacteriaceae species, including Klebsiella pneumoniae, Escherichia coli and Enterobacer cloacae to theorise. The blaNDM genetic surrounding as well as plasmid structure was evaluated in order to describe blaNDM acquisition by IncA/C and IncFII plasmid as well as other genetic mobilisation of resistance mechanisms. This was achieved via next generation sequencing and bioinformatic analysis through both the Illumina platform and CLC Genomics workbench. The blaNDM genetic context in all isolates consisted of a truncated Tn125 structure carrying blaNDM with a native IS element upstream e.g. ISKpn14, regardless of blaNDM variant, plasmid backbone type and bacterial species. This may infer that plasmids capable of high conjugation rates have acquired blaNDM via transposition of a truncated Tn125, which could have provided the initial platform for blaNDM’s rapid dissemination to multiple bacterial species within the Enterobacteriaceae family.