Biodegradable nanoparticles can be effective antigen carriers and immune adjuvants for DNA vaccines. We further investigated the incorporation of nanomagnets and magnetic fields, to enhance the efficiency of malaria blood-stage vaccines. Dendritic cells (DC) transfection and activation by DNA vaccines, would be able to increase their ability to stimulate T cells. To achieve this, we formulated magnetic nanovectors comprising polyethyleneimine (PEI)-coated superparamagnetic iron oxide nanoparticles (SPIONs), formulated with/without further hyaluronic acid (HA) polymers of different molecular weights (<10 kDa and 900 kDa). The later polymer can reduce cytotoxicity, and facilitate endocytosis of particles into DCs via specific surface receptors, as well as promote DC maturation. DNA encoding Plasmodium yoelii merozoite surface protein MSP1-19 and a plasmid encoding yellow fluorescent gene (YFG) were added to the magnetic complexes at various HA:PEI ratios to make the nanoplexes. These nanoplexes were able to transfect and mature DC in vitro. Vectors containing the highest molecular weight HA yielded superior transfection efficiency. Moreover, magnetic fields significantly enhanced DC transfection and maturation. The above formulations were tested in vivo as malaria nanovaccines via different routes of administration (intraperitoneal, intramuscular and subcutaneous), further with/without the influence of a magnetic field at the site of injection, to promote an antigen depot effect. The results confirmed in vitro findings, with up-regulation of CD86 and increased DC transfection in vivo after immunization, and the induction of high levels of antibodies, which were further increased by using a magnetic field during immunization. The subset of T cells (Th1, Th17 or Th2) induced by these nanovaccines was influenced both by the route of immunization and the application of a magnetic field . High IFN gamma and IL17 producing CD4 T cells to MSP1-19 were further associated with highly efficient induction of MSP1-19-specific cytophilic antibodies.