As a first human oncogenic virus, Epstein-Barr virus (EBV) infection is associated with many human malignancies, such as Nasopharyngeal carcinoma (NPC) and post-transplant lymphoproliferative disease (PTLD). In vitro, EBV can immortalize primary B lymphocytes into lymphoblastoid cell line (LCL). Like B cell receptor activation in B cells, EBV infection may trigger protein tyrosine kinase (PTK) activation, which plays crucial roles in outside-in stimuli. In our study, we utilized kinase display to reveal the kinase expression profiles in primary B cells and LCL. The exhibition of differential expression of PTK in these two kinds of cells provides the clues for signaling elicited by EBV infection and immortalization. As expected, a panel of well-known PTK is expressed in both B cells and LCL, such as Lyn and Syk. Of note, we find that recepeur d’origine nantais (RON) is expressed in LCL but not in primary B cells. The expression and regulation mechanism of RON in EBV-infected cells would be investigated in this study. Mechanistically, the viral latent membrane protein LMP1 conducts this up-regulation via the NF-kB signaling pathway. The expression and biological significance of RON in the EBV-associated NPC and PTLD would also be conducted in this study.